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KMID : 0379120160440030201
Korean Journal of Mycology
2016 Volume.44 No. 3 p.201 ~ p.205
Occurrence of Leaf Spot Caused by Stemphylium lycopersici on Cirsium setidens in Korea
Choi Hyo-Won

Kim Seok-Gu
Hong Sung-Kee
Lee Young-Kee
Lee Jae-Geum
Kim Hyo-Won
Lee Eun-Hyeong
Abstract
In August 2015, leaf spot symptoms were observed on Korean gondre thistle () in Youngwol, Korea. During the early stage, the symptoms appeared as one or more small gray-brown to brown spots on plant leaves. The spots showed extensive enlargement over time and eventually became large dark brown to black lesions on the whole leaf. species were consistently isolated from affected leaves. All isolates were identified as , , or based on morphological and cultural characteristics. The isolates were confirmed as based on a multilocus sequence analysis using the ribosomal internal transcribed spacer (ITS) region, elongation factor 1, GAPDH (glyceraldehyde-3-phosphate dehydrogenase), and the noncoding region between the vacuolar membrane ATPase catalytic subunit A gene and a gene involved in vacuolar biogenesis. Pathogenicity was tested by spore suspension inoculation on wounded or unwounded gondre leaves. The lesions were observed on inoculated leaves within 3 days after inoculation, regardless of wound. To our knowledge, this is the first report of the leaf spot on gondre thistle caused by in Korea or elsewhere.
KEYWORD
Gondre, Leaf spot, Stemphylium lycopersici
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